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目的 研究肿瘤坏死因子 (TNF)、5 氟脲嘧啶 ( 5 Fu)、丝裂霉素 (MMC )单用及TNF与 5 Fu、MMC不同配伍方案对人胃癌细胞SGC 790 1的细胞毒作用。方法 应用噻唑蓝比色法 (MTT )测量各实验组的光密度值 (OD值 ) ,计算杀伤率。结果 单独应用各药时 ,三者对SGC 790 1均有细胞毒作用 ,TNF与 5 Fu、MMC配伍时细胞毒作用明显增强 (P <0 .0 1) ,不同配伍方案存在非常显著的差异 (P <0 .0 1) ,以先给化学药物、后加TNF组毒性作用最强。结论 TNF能成倍提高化学药物的细胞毒作用 ,且应在先使用化学药物的基础上给药。
Objective To study the cytotoxic effect of different combinations of TNF, 5 Fu, MMC and MMC with 5 Fu and MMC on human gastric cancer cell line SGC 790 1. Methods The optical density (OD) of each experimental group was measured by MTT method and the kill rate was calculated. Results All the three drugs had cytotoxic effect on SGC 7901. When cytotoxicity of TNF and 5 Fu, MMC was significantly enhanced (P <0.01), there were significant differences among different compatibility regimens P <0. 01), to give chemical drugs, followed by the toxicity of TNF group strongest. Conclusions TNF can double the cytotoxic effect of chemical drugs and should be administered on the basis of chemical drugs.