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目的:观察不同氧环境下中药罗勒多糖对人卵巢癌细胞株SKOV3生物学行为的影响,探讨其抗卵巢癌作用的可能机制。方法:罗勒多糖分别在常氧(21%O2、5%CO2)和乏氧(1%O2、5%CO2和94%N2)环境中作用于人卵巢癌细胞株SKOV3,形态学观察不同氧环境下各组细胞形态差异;细胞凋亡-Hoechst33258染色检测药物对人卵巢癌细胞凋亡情况的影响;MTT法检测各组细胞增殖能力的变化。结果:与对照组相比,乏氧环境下罗勒多糖能够促进人卵巢癌细胞株SKOV3的凋亡,抑制SKOV3细胞增殖,而常氧环境下罗勒多糖作用相反。结论:罗勒多糖在常氧及乏氧环境下对人卵巢癌细胞株SKOV3生物学行为的影响效应截然相反,提示抗肿瘤药物及生物疗法的体外机制研究设计应充分考虑氧环境的影响。
Objective: To observe the effect of basilar polysaccharide on the biological behavior of ovarian cancer cell line SKOV3 under different oxygen environment and to explore its possible mechanism of anti-ovarian cancer. METHODS: Basil polysaccharide was administered to human ovarian cancer cell line SKOV3 under normoxia (21% O2, 5% CO2) and hypoxia (1% O2, 5% CO2 and 94% N2) The morphological changes of cells in each group were observed. The apoptosis was detected by Hoechst33258 staining and the apoptosis of human ovarian cancer cells was detected by MTT assay. Results: Compared with the control group, basil polysaccharide could promote the apoptosis of human ovarian cancer cell line SKOV3 and inhibit the proliferation of SKOV3 cells under the hypoxia condition, while basal polysaccharide had the opposite effect under the normoxic condition. CONCLUSION: The effect of basilain on the biological behavior of human ovarian cancer cell line SKOV3 under normoxia and hypoxia is completely opposite, suggesting that the in vitro mechanism of antitumor drug and biological therapy should fully consider the influence of oxygen environment.