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目的 探讨痢疾菌苗滴鼻免疫后，小鼠不同粘膜部位和其它部位免疫应答的影响。方法将Ｂａｌｂ／ｃ小鼠随机分为３组，每组１５只。舰疾菌苗株 ＴＩＭ－２１１７或 ＦＳ－５４１６，按 ５×10～6、１×１０～７、４×１０～７和４×１０～７ＣＦＵ／只滴鼻跟 Ｂａｌｂ／ｃ小鼠４次涧隔两 ｗｂ。分离鼻相关淋巴组织（ＮＡＬＴ）、鼻通道（ＮＰ）及脾、小肠的ＰＰ结淋巴细胞。一部分细胞采用流式细胞术检测淋巴细胞表型的变化；另一部分细胞与全菌破碎抗原共培养，于不同时间点取出，提取ＲＮＡ做ＲＴ－ＰＣＲ。结果两株菌苗经鼻内免疫后，ＮＡＬ、ＮＰ和ＰＰ结的淋巴细胞中ＣＤ３Ｔ细胞显著增加，其中以ＣＤ４＋Ｔ细胞增加为主。ＴＳＭ－２１１７株免疫组的脾细胞中，Ｂ２２０＋细胞显著增加；而ＦＳ－５４１６株免疫组的脾细胞中，ＣＤ３＋Ｔ细胞显著增加。免疫小鼠的ＮＡＬＴ、ＮＰ和ＰＰ结淋巴细胞，在体外经抗原刺激后，均在不同时间出现ＩＦＮ－γ和ＩＬ-４ｍＲＮＡ的表达，ＴＧＦ－β ｍＲＮＡ的表达在免疫前后无明显变化。结论痢疾菌苗经鼻粘膜免疫，可诱导不同粘膜部位及全身免疫应答的产生。
Objective To investigate the effect of dysentery vaccine on the immune response of different mucosal parts and other parts of mice after intranasal immunization. Methods Balb / c mice were randomly divided into three groups of 15. Ship disease vaccine strain TIM-2117 or FS-5416, according to 5 × 10 ~ 6, 1 × 10 ~ 7,4 × 10 ~ 7 and 4 × 10 ~ 7CFU / nasal drops with Balb / c mice 4 times stream Every two wb. Nasal-associated lymphoid tissue (NALT), nasal passages (NP), and PP-knot lymphocytes from the small intestine were isolated. A part of cells were detected by flow cytometry lymphocyte phenotype changes; the other part of the cells were co-cultured with whole-cell antigen, at different time points removed, RNA extraction for RT-PCR. Results After the two strains of mice were immunized intranasally, CD3 T cells were significantly increased in lymphocytes of NAL, NP and PP, with the increase of CD4 + T cells. The splenocytes of TSM-2117 immunized group showed a significant increase of B220 + cells, while the splenocytes of FS-5416 immunized group showed a significant increase of CD3 + T cells. Immunization of mice with NALT, NP and PP junction lymphocytes in vitro after antigen stimulation, IFN-γ and IL-4 mRNA expression at different times, TGF-β mRNA expression before and after immunization did not change significantly. Conclusion Dampness vaccine through nasal mucosal immunization, can induce different parts of mucosal and systemic immune response.