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目的:探讨多药耐药相关蛋白(MRP)基因的表达与食管贲门癌临床病理特征的关系。方法:应用逆转录多聚酶链反应(RT—PCR)方法,对83例食管贲门癌组织、癌旁及45枚淋巴结中MRP基因的表达进行检测分析。结果:83例食管癌贲门中MRP阳性19例(22.9％),癌旁组织MRP阳性3例(3.6％),癌组织的MRP阳性率与癌旁组织比较具有非常显著性差异(P<0.005)。26枚转移淋巴结中MRP阳性6例(23.1％),19枚非转移淋巴结中未见MRP阳性表达;MRP表达阳性与肿瘤大小及淋巴转移与否无明显关系,但TNMⅢ、Ⅳ期和肿瘤侵犯深肌层以上的MRP基因表达增高,并多分布于低分癌中。48例患者随访发现,MRP阳性者,肿瘤复发、转移的发生率高于MRP阴性者(P<0.05)。结论:食管、贲门癌组织中MRP基因表达增高,与转移淋巴结组织的MRP基因表达具有一致性,MRP的过度表达除了表现肿瘤的多药耐药外,还可能提示这些患者预后不良。
Objective: To investigate the relationship between multidrug resistance-related protein (MRP) gene expression and clinical and pathological features of esophageal and cardial carcinoma. Methods: The expression of MRP gene in 83 cases of esophageal and cardial cancers, adjacent tissues and 45 lymph nodes were detected by reverse transcription polymerase chain reaction (RT-PCR). Results: In 83 cases of esophageal cancer, MRP was positive in 19 cases (22.9%) and paracancerous tissues in 3 cases (3.6%). The positive rate of MRP in cancer tissue was significantly different from that in paracancerous tissues (P <0.005) . There were 6 positive cases (23.1%) of MRP positive in 26 metastatic lymph nodes and no positive MRP in 19 non-metastatic lymph nodes. The positive expression of MRP was not related to tumor size and lymphatic metastasis, but TNMⅢ and Ⅳ tumor invasion depth MRP gene expression above the muscular layer increased, and more in low-grade cancer. 48 patients were followed up found that MRP positive, tumor recurrence and metastasis was higher than the incidence of MRP negative (P <0.05). Conclusion: The expression of MRP gene in esophageal and gastric cardia cancer tissues is increased, which is consistent with MRP gene expression in metastatic lymph node tissues. In addition to the multidrug resistance of tumors, MRP overexpression may also indicate poor prognosis in these patients.