论文部分内容阅读
Ska2(spindle and kinetochore associated complex subunit 2),又称FAM33A(family with sequence similarity 33,member A),是新近发现的一个与细胞周期调控和肿瘤发生发展紧密相关的基因,且与该团队前期发现的新基因PRR11(proline rich 11)共享一个双向启动子。但是,Ska2在肺癌中的具体作用和分子机制仍不清楚。该研究选用肺癌细胞系H1299,采用RNAi技术构建Ska2基因沉默的稳定细胞株,并进行了细胞表型和潜在分子机制分析。RT-PCR和Western blot结果表明,Ska2在mRNA和蛋白质水平上的表达均被有效抑制。细胞增殖、细胞迁移和侵袭实验结果表明,与对照细胞相比,Ska2基因沉默稳定细胞株的细胞增殖能力、细胞迁移和侵袭能力均显著降低。此外,Ska2基因被沉默后,CCNA1基因的表达显著下调。该研究的结果提示,Ska2与其对侧基因PRR11的功能高度相关,可能与PRR11共同参与肺癌细胞增殖、迁移和侵袭行为的调节。
Ska2, also known as FAM33A (family with sequence similarity 33, member A), is a recently discovered gene closely related to cell cycle regulation and tumorigenesis. The new gene PRR11 (proline rich 11) shares a bidirectional promoter. However, the specific role and molecular mechanisms of Ska2 in lung cancer remain unclear. The study selected lung cancer cell line H1299, the use of RNAi technology Ska2 gene silencing stable cell lines, and cell phenotype and potential molecular mechanism analysis. The results of RT-PCR and Western blot showed that Ska2 mRNA and protein levels were effectively inhibited. The experimental results of cell proliferation, cell migration and invasion showed that Ska2 gene silencing stable cell lines significantly reduced cell proliferation, cell migration and invasion compared with control cells. In addition, the expression of CCNA1 gene was significantly down-regulated after Ska2 gene silencing. The results of this study suggest that Ska2 is highly correlated with the function of its contralateral gene PRR11 and may be involved in the regulation of proliferation, migration and invasion of lung cancer cells in combination with PRR11.