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目的:建立核桃楸药用部位总黄酮成分含量测定方法,明确其变化规律。方法:采用75%乙醇回流对青龙衣中总黄酮类成分进行提取,以芦丁为对照品,采用硝酸铝-亚硝酸钠比色法,AB-8大孔树脂除色素,建立含量测定方法。结果:初伏前后5个采集时间青龙衣总黄酮含量分别为3.15,3.49,3.90,3.98,3.96 mg.g-1。结论:初伏前黄酮总含量较低,初伏后总黄酮含量呈逐步增加的趋势,并且有一个快速增长的过程,伏天过后含量略有下降;不同药用部位黄酮含量顺序为叶>皮>茎。
Objective: To establish a method for the determination of the content of total flavonoids in medicinal parts of Juglans mandshurica and clarify its changing rules. Methods: The content of total flavonoids in Qinglongyi was extracted by refluxing with 75% ethanol. Rutin was used as reference substance, and the determination method was established by using aluminum nitrate - sodium nitrite colorimetric method and AB-8 macroporous resin. Results: The content of total flavonoids in Qinglongyi before and after initial incubation were 3.15, 3.49, 3.90, 3.98 and 3.96 mg.g-1, respectively. CONCLUSION: The content of total flavonoids is low before the initial incubation and the content of total flavonoids gradually increases after the initial incubation, and there is a rapid growth process. The content of flavonoids decreases slightly after the first day; the order of flavonoids in different medicinal parts is leaf> stem.