Studies on Reaction Mechanism Between Sparfloxacin and Bovine Serum Albumin

来源 :高等学校化学研究(英文版) | 被引量 : 0次 | 上传用户:aileenliuwei
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The binding of sparfloxacin and bovine serum albumin(BSA) in aqueous solution was studied by means of fluorescence and absorbance spectra, and the interactions influenced by Fe3+ and Cu2+ were explored. Based on the Scatchard′s site binding model and fluorescence quenching, practical formulas for a small molecule ligand attaching to a bio-macromolecule are proposed. The binding parameters were measured according to the suggested models, and the binding distance, the transfer efficiency of energy between sparfloxacin and BSA were obtained in view of the Frster theory of non-radiation energy transfer. The effect of sparfloxacin on the conformation of BSA was analyzed by means of synchronous fluorescence spectroscopy.
其他文献
A system for the numerical simulation of 3D temperature and stress fields during casting process was studied, developed and practiced based on finite differenc
Some chiral binaphthyl crown ethers were synthesized. The anionic polymerization of methyl methacrylate (MMA) was carried out in the presence of t-BuOK, Ph2CHK
Here the authors are interested in the zero set of Sobolev functions and functions of bounded variation with negative power of integrability. The main result is
A series of monometallic nitrides and bimetallic nitrides were prepared by temperature-programmed reaction with NH3. The effects of Co, Ni and Fe additives and
The cellular neural/nonlinear network (CNN) is a powerful tool for image and video signal processing,robotic and biological visions. This paper discusses a gene
The synthesis of a new type of polymers with main chain chirality based on BINOL skeleton is described. Titanium-BINOLate catalysts are easily generated from th
Trial function method is applied to solve generalized mKdV (GmKdV for short) equations. It is shown that GmKdV equations with a realnumber parameter can be solv
The title complex NiL(py)3, where H2L = N-salicylaldehyde-N'-phenoxyacetyl hydrazine, was prepared and characterized by X-ray diffraction. The single crystal of
The experimental investigation demonstrates that a satisfactory result can be expected for pressure swing adsorption (PSA) purification of natural gas as raw ma
Octaphenylsilsesquioxane(OPhS) was prepared by a modifying method and a new core-shell nanocomposite, octa(2,4-dinitrophenyl)silsesquioxane, [(R2PhSiO1.5)8, R=-