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目的 探讨胆必清颗粒对实验性急性细菌性胆管炎保护作用的机理。方法 经胆管内注射 1mL大肠杆菌 ( 1× 10 8cfu·mL)引发急性细菌性胆管炎。采用硝酸还原法测定血清NO水平 ;酸滴定法测定PLA2 活性 ;放免法测定血清TNF α ,IL 6和血浆TXB2 和PGF1α水平。结果 与对照组比较 ,模型组血清NO、PLA2 、TNF和血浆TXB2 水平均明显升高 (P <0 .0 5 ) ;各胆必清治疗组的上述指标均显著降低 (P <0 .0 1)。结论 胆必清颗粒能明显抑制前炎性因子PLA2 和炎性细胞因子TNF α ,IL 6的过量产生 ,从而进一步揭示了该方剂的抗炎和屏障保护作用机理。
Objective To explore the protective mechanism of bixiqing granule on experimental acute bacterial cholangitis. Methods Acute bacterial cholangitis was induced by bile duct injection of 1 mL of E. coli (1 × 10 8 cfu · mL). The level of serum NO was determined by nitric acid reduction method. The activity of PLA2 was determined by acid titration. The levels of TNFα, IL-6, TXB2 and PGF1α in serum were determined by radioimmunoassay. Results Compared with the control group, the levels of serum NO, PLA2, TNF and plasma TXB2 in the model group were significantly increased (P <0.05), and the above indexes in each group were significantly decreased (P <0.01 ). Conclusion GCD can significantly inhibit the overproduction of proinflammatory cytokines PLA2 and inflammatory cytokines TNFα and IL 6, further revealing the anti-inflammatory and barrier protective mechanisms of this formula.