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对β- 葡糖苷酸酶(GUS)基因在转基因花生T1- T3 代植株中的活性测定和NPT II基因在T3 代植株中的活性和PCR检测表明:T1 代的GUS基因为1 ∶1、3∶1 等分离, T2 和T3 代GUS基因符合3 : 1 单基因显性遗传规律的植株数增加,1 : 1 分离单株比例逐渐减少。从T3 代中就可选择到GUS基因纯合的单株,得到了4 株遗传性稳定的单株,说明GUS基因在转基因花生植株后代的遗传应属于单基因显性遗传规律。实验证明,位于同一质粒上的GUS和新霉素磷酸转移酶(NPT II)基因,在转基因花生植株中表现为连锁遗传。
The activity of β-glucuronidase (GUS) gene in transgenic T1-T3 transgenic peanut plants and the activity of NPT II gene in T3 plants were detected by PCR. The results showed that the GUS genes in T1 generation were 1: 1, 3 : 1 and other isolates. The number of GUS gene conformed to the 3: 1 single gene dominant inheritance rule increased in T2 and T3 generations, and the proportion of 1: 1 isolated plants decreased gradually. From the T3 generation, we could select the single plant with homozygous GUS gene and obtained 4 hereditary stable plants, indicating that the inheritance of the GUS gene in the transgenic peanut plants should belong to the single gene dominant inheritance rule. Experiments show that GUS and neomycin phosphotransferase (NPT II) genes located on the same plasmid behave as the linkage inheritance in the transgenic peanut plants.