论文部分内容阅读
用ＭｃＡｂ－Ｓａｎｄｗｉｃｈ－ＥＬＩＳＡ（夹心法）及ＭｃＡｂ－Ｄｏｔ－ＥＬＩＳＡ（点免疫法）同步检测了４６例包虫病人及４２例正常人的不同抗原样品，结果表明：ＭｃＡｂ夹心法对患者血清、尿液抗原阳性检出率分别为：８０．４３％、７８．２６％，明显高于对照组（Ｐ＜０．０５），且血清与尿液抗原检测结果无差别（Ｐ＞０．０５），具有相关性（Ｐ＜０．０５）；点免疫法抗原检出率分别为７３．９１％、５８．７９％。两法在检测尿液抗原上有显著性差异（Ｐ＜０．０５）。提示前法优于后法。推荐检测尿液抗原（夹心法）进行包虫病的无损伤性免疫诊断。
The different antigens of 46 hydatid and 42 normal individuals were detected synchronously by McAb-Sandwich-ELISA (sandwich method) and McAb-Dot-ELISA (dot immunoassay). The results showed that McAb sandwich method could detect the serum, The positive rate of urine antigen was 80.43% and 78.26%, respectively, which was significantly higher than that of the control group (P <0.05). There was no difference between the serum and urine antigen (P> 0.05) (P <0.05). The detection rates of spot immunization antigen were 73.91% and 58.79%, respectively. Two methods in the detection of urine antigen significant difference (P <0.05). Tips before the law is better than the latter method. Recommended urine test antigen (Sandwich method) for echinococcosis non-invasive immune diagnosis.