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[目的]探讨早期胃印戒细胞癌内肿瘤干细胞的分布和定位。[方法]采用组织化学和免疫组织化学染色技术,检测47例早期胃印戒细胞癌的黏液分泌特点、分化增殖特性和干细胞标志物的表达。[结果]早期胃印戒细胞癌具有特征性的“分层结构”:浅表层为典型印戒细胞,第二层为低分化癌细胞,少数病例(4例)含第三层癌细胞,呈泡沫样。浅表层及第三层癌细胞具有黏液分泌功能,主要表达MUC5AC和MUC6,而不表达干细胞标志物。第二层癌细胞无黏液分泌功能,不表达分化标志物,Sox2、musashi-1、hTERT干细胞标记阳性,Ki-67阳性率为28.6%±4.3%。[结论]早期胃印戒细胞癌的肿瘤干细胞定位于分层结构的第二层,可能与肿瘤的早期进展有关。
[Objective] To investigate the distribution and localization of tumor stem cells in early gastric signet ring cell carcinoma. [Methods] The histological and immunohistochemical staining techniques were used to detect muco-secretion characteristics, differentiation and proliferation characteristics and expression of stem cell markers in 47 cases of early gastric signet-ring cell carcinoma. [Results] The early gastric signet-ring cell carcinoma has the characteristic “stratified structure”: the superficial layer is a typical signet ring cell, the second layer is a poorly differentiated cancer cell, and a few cases (4 cases) , Was foamy. The superficial and the third layer cancer cells have mucus-secreting functions, mainly expressing MUC5AC and MUC6, but not stem cell markers. The second layer of cancer cells had no mucus secretion function and no differentiation markers. Sox2, musashi-1 and hTERT stem cells were positive, and the positive rate of Ki-67 was 28.6% ± 4.3%. [Conclusion] Cancer stem cells of early gastric signet-ring cell carcinoma locate in the second layer of the hierarchical structure, which may be related to the early progress of the tumor.