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本文初步报告了用华枝睾吸虫成虫石蜡切片抗原,通过间接荧光抗体试验(IFAT)检测华枝睾吸虫病人(粪检虫卵阳性)血清50份,44份(88%)呈阳性反应,健康人血清85份,7例阳性,假阳性率为8.23%。表明,成虫石蜡切片IFAT检测华枝睾吸虫病人抗体,具有一定的敏感性与特异性。石蜡切片制作简便,不需复杂的设备,易于保存,便于携带。因此应用成虫石蜡切片抗原进行IFAT诊断华枝睾吸虫病可能成为有一定实用价值的新方法。
In this paper, we report initially the paraffin-embedded antigens of adult testes of C. sinensis and 50 samples of seminal hoofed sinew (positive for septic jellyfish) were detected by indirect fluorescent antibody test (IFAT), 44 (88%) were positive and healthy Serum of 85 people, 7 cases were positive, the false positive rate was 8.23%. It showed that adult paraffin section IFAT detection of Clonorchis sinensis patient antibody, has a certain sensitivity and specificity. Paraffin sections are easy to make and do not require complicated equipment for easy storage and easy to carry. Therefore, the application of adult paraffin section antigen for IFAT diagnosis of Clonorchiasis may become a new practical method of value.