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本实验研究选用出生并生长在西安(海拨397M)、经标化的雄性Wistar大鼠20只,随机分为二组,一组(对照组)在出生地取材,另外一组(缺氧组)急进入高原医学实验站(当地海拨3700M),用原居住地饲料喂养存活四周.经麻醉、生理盐水、戊二醛磷酸缓冲液灌注固定,开颅取脑,再用戊二醛固定,取顶叶2、3区皮质,切成组织块,每只动物取3块,锇酸固定,常规脱水包理.经超薄切片机切片,醋酸铀和枸橼酸铅双染后,在电镜
In our study, 20 male Wistar rats, born and growing in Xi’an (at an altitude of 397M), were randomly divided into two groups. One group (control group) was drawn from the place of birth and the other group (hypoxic group ) Into the plateau medical experiment station (local high-altitude 3700M), feed with the original living place to survive for four weeks by anesthesia, saline, glutaraldehyde phosphate buffer perfusion, brain craniotomy, and then fixed with glutaraldehyde, Take parietal 2,3 cortical area, cut into tissue pieces, each animal take 3, osmium acid fixed, conventional dehydration package.Ultrasound slicer, uranyl acetate and lead citrate double staining, electron microscopy