目的 :研究肾舒胶囊对人系膜细胞 (HMC)增殖及分泌肿瘤坏死因子 -α(TNF -α)和纤连蛋白 (FN)的影响。方法 :采用血清药理学方法 ,将肾舒胶囊含药血清加入培养的HMC中 ,MTT法测定HMC增殖情况 ,ELISA法测定HMC分泌TNF -α及FN情况。结果 :肾舒胶囊含药血清可明显抑制HMC增殖及其分泌TNF -α和FN ,且其抑制作用呈一定的浓度依赖性和时间依赖性。结论 :肾舒胶囊可能通过抑制MC增殖及其分泌TNF -α等炎症介质和FN等细胞外基质 (ECM)防治肾小球硬化 Objective: To investigate the effects of Shenshu capsule on proliferation and secretion of tumor necrosis factor-α (TNF-α) and fibronectin (FN) in human mesangial cells (HMCs). Methods: Serum pharmacological methods were used to add Shenshu capsule containing serum to cultured HMC. MTT assay was used to measure the proliferation of HMC. TNF-α and FN secreted by HMC were measured by ELISA. Results: Shenshu capsule containing serum could obviously inhibit the proliferation of HMC and the secretion of TNF-α and FN, and the inhibitory effect was in a concentration-dependent and time-dependent manner. Conclusion: Shenshu Capsule may prevent glomerulosclerosis by inhibiting the proliferation of MC and its secretion of TNF-α and other inflammatory mediators and extracellular matrix (FN) such as FN