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利用BALB/c小鼠腹水大量制备抗大田软海绵酸(Okadaic Acid,OA)的单克隆抗体,并以此抗体为探针建立了检测鳍藻毒素(Dinophysistoxins,DTXs)DTX1和DTX2的间接竞争酶联免疫吸附分析方法(Enzyme Linked Immunosorbent Assay,ELISA)。DTX1和DTX2各组分的标准曲线在一定范围内都有良好的线性范围和相关系数,最低检出浓度为0.53μg/L和0.45μg/L;批内和批间平均变异系数为6.18%、5.11%和7.28%、5.79%;加标样品平均回收率为76.4%、79.9%;OA与DTX1和DTX2的交叉反应率分别为53%和79%。结果表明,该方法可用于贝类样品中DTX1和DTX2的残留检测。
A monoclonal antibody against Okadaic Acid (OA) was prepared in large quantities in ascites of BALB / c mice, and indirect antibodies against DTX1 and DTX2 of dinophysistoxins (DTXs) were constructed using this antibody as a probe Enzyme Linked Immunosorbent Assay (ELISA). The calibration curves of DTX1 and DTX2 showed a good linearity and correlation coefficient within a certain range. The minimum detectable concentrations were 0.53μg / L and 0.45μg / L, respectively. The average coefficient of variation (CV) was 6.18% 5.11% and 7.28%, 5.79% respectively. The average recoveries of spiked samples were 76.4% and 79.9%, respectively. The cross-reactivity rates of OA with DTX1 and DTX2 were 53% and 79%, respectively. The results show that the method can be used for the detection of residues of DTX1 and DTX2 in shellfish samples.