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目的:观察高迁移率族蛋白B1(HMGB1)在人牙髓细胞(Human dental pulp cells,hDPCs)中的表达,以及对hDPCs增殖和迁移能力的影响。方法:采用组织块培养法,培养原代hDPCs,取第3～6代细胞用于实验。免疫荧光检测HMGB1在hDPCs中的表达及定位;分别用含不同质量浓度(0.1 ng/mL、1 ng/mL、10 ng/mL、50 ng/mL、100ng/mL、500 ng/mL、1000 ng/mL)HMGB1的培养液培养hDPCs,5天后采用CCK-8法检测细胞增殖能力;细胞划痕实验法观察1 ng/mL质量浓度HMGB1对hDPCs迁移能力的影响。结果:HMGB1表达在hDPCs胞核;低浓度HMGB1(0.1 ng/mL、1 ng/mL、10 ng/mL、50 ng/mL、100 ng/mL)明显促进细胞增殖;1 ng/mL HMGB1明显促进hDPCs迁移能力。结论:HMGB1表达在正常hDPCs胞核中,细胞外低浓度HMGB1可以促进细胞增殖和迁移。
OBJECTIVE: To observe the expression of high mobility group box 1 (HMGB1) in human dental pulp cells (hDPCs) and their effects on the proliferation and migration of hDPCs. Methods: The primary cultured hDPCs were cultured by tissue culture method and the 3rd to 6th generation cells were used for the experiment. Immunofluorescence was used to detect the expression and localization of HMGB1 in hDPCs. The expression of HMGB1 in hDPCs was detected by immunohistochemistry. / mL) HMGB1 culture medium were cultured hDPCs, 5 days after the cell proliferation assay using CCK-8 method; cell scratch test method of 1 ng / mL mass concentration of HMGB1 hDPCs migration ability. Results: HMGB1 was expressed in the nucleus of hDPCs. Low concentrations of HMGB1 (0.1 ng / mL, 1 ng / mL, 10 ng / mL, 50 ng / mL, 100 ng / mL) hDPCs migration ability. Conclusion: HMGB1 expression in normal hDPCs nuclei, extracellular low concentrations of HMGB1 can promote cell proliferation and migration.