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本文主要报道宣肺解毒颗粒剂对小鼠细胞免疫功能的作用。用宣肺解毒颗粒剂水溶液灌胃1天后,以二硝基氟苯(DNFB)按常规致敏Balb/c小鼠,再连续给药5天后,于耳廓部发敏,36h后,用7mm直径打孔器分别切取耳片,称量,实验侧与对照侧耳片重量之差即为耳肿胀度,以此示小鼠迟发超敏反应程度。同时,取各组小鼠脾脏,按常规做体外脾细胞增殖实验,比较脾细胞及在ConA或LPS刺激下细胞增殖程度(用MTT法)。并用成淋巴细胞法测各组脾细胞体外诱生IL-2的能力。结果表明宣肺解毒颗粒剂12.8g/kg·d~(-1)和32g/kg·d~(-1)剂量组与生理盐水对照组
This article mainly reported the effect of Xuanfei Jiedu Granules on the cellular immune function in mice. After intragastric administration with a solution of Xuanfei Jiedu granules for 1 day, Balb/c mice were sensitized with dinitrofluorobenzene (DNFB) in a routine manner, and after 5 consecutive days of administration, they were allergic to the auricular region. After 36 hours, 7 mm was used. Diameter punches were used to cut out the ears and weighed. The difference between the weight of the experimental side and the control side ear was the degree of ear swelling, indicating the degree of delayed hypersensitivity in mice. At the same time, the spleens of mice in each group were taken and routinely performed splenocyte proliferation experiments in vitro to compare the spleen cells and the degree of cell proliferation under the stimulation of ConA or LPS (using MTT assay). Lymphocyte assay was used to measure the ability of spleen cells to induce IL-2 in vitro. The results showed that Xuanfei Jiedu Granules 12.8g/kg·d~(-1) and 32g/kg·d~(-1) dose group and physiological saline control group