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桑椹菌核病是影响桑椹产量和品质的主要病害,核盘菌(Sclerotinia sclerotiorum)是一种新发现的疑似桑椹菌核病的病原真菌.以果桑品种大10为供试材料,利用数字基因表达谱技术,筛选核盘菌侵染相关的差异表达基因(DEGs)并分析其功能与代谢途径.在感染和未感染核盘菌的桑树幼果样品之间检测到了显著差异表达基因l 226个(上调表达基因1 088个,下调表达基因138个),其中包含33类172个抗性相关基因,35类112个转录因子基因.生物信息学分析发现,显著差异表达基因显著富集于4个细胞组分(胞外区、细胞壁、外部封装结构、质外体)、6种分子功能(抗氧化活性、酶催化活性、酶调节活性、核酸结合转录因子活性、营养物质代谢、翻译调控活性等)和6个代谢通路(亚油酸代谢、半胱氨酸和蛋氨酸代谢、植物-病原互作、苯丙氨酸代谢、芳香族氨基酸生物合成、糖类次生代谢产物生物合成).选取可能与桑树对核盘菌感染应答相关的F-box等5个基因作为目的基因,进行实时荧光定量PCR检测验证,检测数据与RNA-seq表达谱的分析结果基本一致.研究结果为阐释桑树应答核盘菌侵染的分子机制积累了基础数据.“,”Mulberry is susceptible to mulberry fruit sclerotiniosis which seriously affects the yield and quality of mulberry fruit.Sclerotinia sclerotiorum is a newly discovered pathogenic fungus causing mulberry fruit sclerotiniosis.In this study,a fruit mulberry variety Da10 was used as material and digital gene expression profiling was employed to dissect differentially expressed genes (DEGs) and to analyze their functions and metabolic pathways related to Sclerotinia sclerotiorum infection.As a result,1 226 significantly differentially expressed genes were detected between the infected and non-infected mulberry fruit samples.Among them,1 088 genes were up-regulated and 138 genes were down-regulated.There are 172 genes related to disease resistance (divided into 33 classes) and 112 genes of transcription factors (divided into 35 classes).Through GO functional annotation,the significantly differentially expressed genes were found to enrich significantly in 4 cell components (extracellular region,cell wall,external encapsulating structure,and apoplast),in 6 molecular functions (antioxidant activity,enzyme catalytic activity,enzyme regulatory activity,nucleic acid binding transcription factor activity,nutrient metabolic activity,and translation regulatory activity),and in 6 metabolic pathways (linoleic acid metabolism,cysteine and methionine metabolism,plant-pathogen interaction,phenylalanine metabolism,phenylpropanoid biosynthesis,and biosynthesis of secondary metabolites).Expression profiles of 5 genes related to response of mulberry to Sclerotinia sclerotiorum infection,including F-box,are analyzed by real-time fluorescent quantitative PCR.The results are almost identical with that from RNA-seq.These results accumulate basic data for elucidating molecular mechanism of mulberry in response to infection by Sclerotinia sclerotiorum.