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为深入了解铜绿丽金龟的取食行为,探索开发安全高效的植物源引诱剂,应用昆虫触角电位反应仪和Y型嗅觉仪测试其对不同寄主植物挥发物的趋向行为差异,并根据室内行为结果配制诱剂进行大田试验。结果表明:铜绿丽金龟雄虫对水杨酸甲酯和(1,1’-联环戊基)-2-酮的触角电位反应(electroantennography,EAG)值显著高于其它试剂;石竹烯和水杨酸甲酯能引起雌虫触角较强的电位反应;乙酸顺式-3-己烯酯和石竹烯分别对雄虫和雌虫有较高的嗅觉选择反应率,分别达0.95和0.94。综合EAG和嗅觉试验结果,选择对雌、雄虫均有较好引诱效果的反式-2-己烯醛、乙酸顺式-3-己烯酯、石竹烯、(1,1’-联环戊基)-2-酮和水杨酸甲酯进行田间试验,最终筛选到铜绿丽金龟雄虫的最适引诱剂为每诱芯360 mg乙酸顺式-3-己烯酯,雌虫的最适引诱剂为每诱芯360 mg石竹烯,日诱虫量分别可达33.00±1.53头和29.33±1.45头。
In order to further understand the feeding behavior of the copper beetle, explore the development of a safe and efficient plant attractant, use the tentacles tentative potential tester and the Y-type olfactometer to test the differences in the tendency behavior of volatiles from different host plants, and according to the indoor behavior results Formulation lure for field trials. The results showed that the electroantennography (EAG) values of male beetle to methyl salicylate and (1,1'-dicyclopentanyl) -2-one were significantly higher than those of the other reagents. Caryophyllene and water Methyl salicylate can cause a strong potential response to the female antennae. Cis-3-hexenyl acetate and caryophyllene have higher selectivity of olfactory response to male and female, respectively, reaching 0.95 and 0.94, respectively. Based on the results of EAG and olfactory test, trans-2-hexenal, cis-3-hexenyl acetate, caryophyllene, (1,1'-linked Pentyl) -2-one and methyl salicylate. The optimum attractant for the male Culex palletii was 360 mg / w cis-3-hexenyl acetate per attractant Attract attractant is 360 mg caryophyllene per trap, and the number of trap insects on the day is up to 33.00 ± 1.53 and 29.33 ± 1.45 respectively.