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本文报告了应用￣３２Ｐ和光敏生物素标记克隆的ｐＢＦ４ＤＮＡ，作为探针，以斑点杂交试验，检测海南、云南和山东省的血样。两种标记探检测结果与镜检的符合率分别为恶性疟９８．６％和９６．２％；间日疟９１．６％和８６．７％；正常人血９５．２％和１００％；并可从多种疟原虫标本中，特异地检出恶性疟原虫，显示探针具有恶生疟原虫的种特异性。检测的敏感度为８４个原虫／μｌ血，检测原虫ＤＮＡ可达１０ｐｇ水平。表明探针具有良好的实用性。
In this paper, pBF4 DNA labeled with biotinylated biotin was reported as a probe to detect blood samples from Hainan, Yunnan and Shandong provinces by dot blot hybridization. The coincidence rates of the two kinds of marker detection and microscopic examination were 98.6% and 96.2% of P. falciparum respectively; 91.6% and 86.7% of P. vivax; 95.2% and 100% of normal human blood; The Plasmodium falciparum can also be detected specifically from a wide range of Plasmodium specimens, demonstrating that the probe has the species specificity of Plasmodium falciparum. The sensitivity of the test was 84 protozoa / μl blood, and the detection of protozoa DNA up to 10pg. It shows that the probe has good practicability.