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[目的]了解马鞍山市7类食品中单增李斯特菌污染状况,并评价不同方法的检测效果,为食品中单增李斯特菌定量风险评估提供科学依据。[方法]依据国标GB4789.30-2003和国家食源性疾病监测网2007年年度工作手册进行,同时用EB增菌法、LB增菌法和Fraser肉汤培养法对食品样品进行单增李斯特菌分离鉴定并进行比较。[结果]7类482份食品样品,检出单增李斯特菌(L.m)56株,检出率为11.62%;在288份生肉和水产品中检出28株产H2S李斯特菌,阳性率为9.72%。[结论]在7类食品中除冰淇淋外,均不同程度受到L.m污染,尤以速冻面米食品污染严重,其次为熟肉制品、生畜禽肉和水产品。LB增菌法明显优于EB增菌法,Fraser肉汤培养法和LB增菌法检出率相等,而后者操作更方便。
[Objective] To understand the contamination status of Listeria monocytogenes in seven kinds of food in Ma’anshan city and to evaluate the detection effect of different methods, and provide a scientific basis for quantitative risk assessment of Listeria monocytogenes in food. [Method] According to the national standard GB4789.30-2003 and the National Foodborne Disease Surveillance Network 2007 Annual Working Manual, the food samples were mono-amplified with EB method, LB method and Fraser broth method. Isolates were identified and compared. [Results] 56 strains of Listeria monocytogenes (Lm) were detected in 482 food samples of 7 categories, the detection rate was 11.62%; 28 strains of Listeria monocytogenes were detected in 288 raw meat and aquatic products, the positive rate 9.72%. [Conclusion] L.milk was contaminated to some extent except for ice cream in 7 kinds of foodstuffs, especially the food of frozen noodles was seriously polluted, followed by cooked meat products, live poultry meat and aquatic products. LB enrichment was significantly better than the EB by bacteria, Fraser broth culture and LB by the same detection rate of bacteria, while the latter operation more convenient.