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目的 :探讨一氧化氮 (NO)在肾缺血再灌流过程中的作用。方法 :制作肾缺血再灌流模型 ,检测缺血 60 min,再灌流 1 5min和 2 4 h的肾组织 NO浓度、一氧化氮合酶 (NOS)活性、亚硝酸盐 /硝酸盐浓度 (NO-2 / NO-3 )及丙二醛 (MDA)浓度。结果 :肾缺血 60 min后 NOS活性降低 ,NO水平下降 ,再灌流 1 5min后 NOS活性有所提高 ,再灌流 2 4 h,NOS活性明显提高 ,NO水平提高 ,同时肾损伤逐渐明显 ,肾组织中 NO-2 / NO-3 、MDA明显提高。结论 :NO在缺血再灌流肾损伤过程中起到了非常重要的作用
Objective: To investigate the role of nitric oxide (NO) in renal ischemia reperfusion. Methods: The model of renal ischemia / reperfusion was established. The NO, NOS, NO-, and NO-1 in renal tissue were detected after ischemia 60 min, reperfusion 15 min and 24 h, 2 / NO-3) and malondialdehyde (MDA) concentrations. Results: NOS activity decreased and NO level decreased 60 min after renal ischemia. NOS activity increased after 15 min of reperfusion. NOS activity increased significantly and NO level increased 24 h after reperfusion. At the same time, renal injury gradually increased, NO-2 / NO-3, MDA significantly increased. Conclusion: NO plays a very important role in the process of renal ischemia-reperfusion injury