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牛膝(Achyranthes bidentata Blume)具有补气强肾的功能,我们从其根部提取分离得到一水溶性的寡糖(AbS),它具有显著的增强免疫功能的活性。本文报道AbS的结构研究。用蒸馏水提取牛膝根块中的水溶性成份,经分级沉淀和醇析得到粗制品,将粗制品经葡聚糖凝胶Sephadex G-50和G-25柱层析得到均一的AbS。用蒽酮法测定其含糖量为100%。
Achyranthes bidentata Blume has the function of invigorating qi and strengthening the kidneys. We extracted and isolated a water-soluble oligosaccharide (AbS) from its roots, which has significant immune-enhancing activity. This article reports the structural study of AbS. Water-soluble components of Achyranthes bidentata block were extracted with distilled water, and the crude product was obtained by fractional precipitation and alcoholysis. The crude product was chromatographed on Sephadex G-50 and G-25 columns to obtain uniform AbS. Anthrone method to determine the sugar content of 100%.