应用Dot-ELISA法对丰产鲫败血病病原菌豚鼠气单胞菌CSS-4-2菌株的全菌抗原进行检测,其最低检出水平为105 cells/mL.Dot-ELISA法对人工感染CSS-4-2菌株濒临死亡或有明显症状丰产鲫的肝、脾、肾组织匀浆上清液的阳性检出率为97%;而对未显症状丰产鲫的肝、脾、肾组织匀浆上清液的阳性检出率为25%.同时发现当感染浓度较高(≥109 cells·mL-1)时,攻毒3h后在脾脏中就能检出病原菌. Dot-ELISA method was used to detect the total bacterial antigens of Aeromonas hydrophila CSS-4-2 strain of Carassius auratus, the lowest detectable level of 105 cells / mL.Dot-ELISA method was used to detect CSS- 4-2 strains are dying or have obvious symptoms The positive detection rate of liver, spleen and kidney homogenate supernatant of high-yield crucian carp was 97%; while on the homogenates of liver, spleen and kidney of high-yield crucian carp The positive detection rate of the supernatant was 25% .It was also found that when the infection concentration was higher (≥109 cells · mL-1), the pathogen could be detected in the spleen 3h after the challenge.