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目的探讨单唾液酸神经节苷脂(GM1)对脑缺血再灌注大鼠海马ARMS与CrkL表达的影响。方法96只SD大鼠随机分为:假手术组(Sham,32只)、神经节苷脂治疗组(GM1,32只)、缺血再灌注组(I/R,32只)。GM1治疗组和I/R组依据缺血后再灌注的不同时间点再细分为6 h、12h、24 h、3 d 4个组。应用免疫组织化学方法和Western blot方法检测各组大鼠海马ARMS与CrkL蛋白的表达。结果免疫组织化学方法及Western blot检测发现,假手术组大鼠海马有微量ARMS与CrkL表达,而经过脑缺血再灌注处理后,ARMS与CrkL表达水平在6 h已显著升高,在24h时达到峰值最高,3d时表达较假手术组仍显著升高;与同一时间点的缺血再灌注组大鼠比较,GM1治疗组的ARMS与CrkL蛋白阳性表达亦显著升高(P<0.05)。结论GM1对脑缺血再灌注损伤的神经保护作用可能与上调ARMS与CrkL蛋白的表达相关。
Objective To investigate the effects of monosialoganglioside GM1 on the expression of ARMS and CrkL in hippocampus of cerebral ischemia-reperfusion rats. Methods 96 Sprague-Dawley rats were randomly divided into sham operation group (Sham 32), ganglioside treatment group (GM 32) and ischemia reperfusion group (I / R 32). The GM1-treated group and the I / R group were subdivided into 4 groups at 6 h, 12 h, 24 h, and 3 d according to the different time points after ischemia-reperfusion. Immunohistochemistry and Western blot were used to detect the expression of ARMS and CrkL in hippocampus of rats in each group. Results Immunohistochemistry and Western blot showed that ARMS and CrkL expression in the hippocampus of rats in sham-operated group were significantly increased. However, the expression of ARMS and CrkL in hippocampus of sham operation group increased significantly at 6 h after cerebral ischemic reperfusion, Reaching the peak value at 3 days. The expression of ARMS and CrkL protein in GM1-treated group was significantly higher than that in ischemia-reperfusion group at the same time point (P <0.05). Conclusion The neuroprotective effect of GM1 on cerebral ischemia-reperfusion injury may be related to the upregulation of ARMS and CrkL protein expression.