Bamboo shoots can be harvested at different ages but the data on the changes in nutritional composition with age are scanty. We standardized harvesting age of bamboo shoots in central India to obtain best quality produce with respect to nutritional composition. The shoots harvested on different days (2 20 days after emergence from ground) were analyzed for their nutritional (dietary fibres, carbohydrates, proteins, total phenols, ascorbic acid, sodium, potassium, phosphorus, calcium, magnesium and phenolic acids) and anti nutritional (cyanogen) constituents. A significant variation (at p≤0.5) was observed in the nutritional composition of shoots of Dendrocalamus asper, D. strictus and Bambusa tulda harvested at different days. An overall decrease was observed in proteins and total phenols while dietary fibres and carbohydrates increased with ages. Significant variation (at p≤0.5) was also observed in phenolic acids while minerals did not vary significantly. Results revealed that the optimum harvesting age for D. asper, D. strictus and B. tulda was on 10 14 days, 6 10 days and 10 16 days (after emergence from the ground) respectively. These results can be used to obtain quality bamboo shoots. We standardized harvesting of in shoots in central India to obtain best quality produce with respect to nutritional composition. The shoots harvested on different days ( 2 20 days after emergence from ground) were analyzed for their nutritional (dietary fibers, carbohydrates, proteins, total phenols, ascorbic acid, sodium, potassium, phosphorus, calcium, magnesium and phenolic acids) variation (at p≤0.5) was observed in the nutritional composition of shoots of Dendrocalamus asper, D. strictus and Bambusa tulda harvested at different days. An overall decrease was observed in proteins and total phenols while dietary fibers and carbohydrates increased with ages. Significant variation (at p≤0.5) was also observed in phenolic acids while minerals did not quite significantly. Results showed that th e optimum harvesting age for D. asper, D. strictus and B. tulda was on 10 14 days, 6 10 days and 10 16 days (after emergence from the ground) respectively. These results can be used to obtain quality bamboo shoots.