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目的：探讨利用高剂量（0．8μg/mL）顺铂（cDDP）诱导胃癌顺铂耐药细胞株的建立及其生物学特性。方法采用大剂量冲击法诱导人胃癌耐顺铂细胞株BGC823/cDDP 建立。观察BGC823/cDDP 细胞形态，CCK-8法检测细胞的耐药指数，CCK-8绘制生长曲线，流式细胞仪检测细胞的生长周期，Transwell 法检测细胞侵袭能力的变化，免疫发光法测定细胞培养液中肿瘤标志物CEA、CA-199含量。结果采用大剂量cDDP 冲击方法诱导后， BGC823/cDDP 细胞较BGC823细胞体积略增大，细胞核增大，形状不规则，并可见散在的多核巨细胞，其对顺铂的耐药指数为4．54，耐药细胞系的倍增时间较正常BGC823细胞延长，BGC823/cDDP 细胞G1期细胞增多，而S、 G2期细胞减少，耐药细胞株侵袭力增强（P ＜0．05），耐药细胞培养液中肿瘤标志物CEA、CA-199含量与正常胃癌细胞无明显差别。结论通过大剂量cDDP 冲击方法建立了顺铂耐药胃癌细胞株BGC823/cDDP，耐药细胞与正常细胞的生物学特性不同。“,”Objective To explore the drug-resistant gastric cancer cell line induced by high-dose cDDP and its biologi-cal characteristics.Methods A drug-resistant gastric cancer cell line BGC823/cDDP was established by high-dose cD-DP.The changes of biological characteristics of the cell lines were observed using light microscopy.The resistance in-dex was determined by CCK-8 assay.The cell growth curve was drawn by CCK-8 assay.The cell cycle of drug-resist-ant cells was tested by flow cytometry.The cell invasion ability was observed by Transwell assay.The densities of CEA and CA-199 in the cell culture medium were evaluated by immune luminescence.Results Compared with BGC823 cells, the BGC823/cDDP cells turned to be slightly bigger and irregular in shape, and some giant cells could be seen. The proliferation rate of BGC823/cDDP cell prolonged, with the resistance index being 4.54.The population doubling-time prolonged.The cell numbers of G1 phase increased while decreased in S and G2 phase in BGC823/cDDP cell.The invasion ability was stronger in BGC823/cDDP cell (P<0.05).The densities of CEA and CA-199 had no significant differences between BGC823 and BGC823/cDDP.Conclusion The cDDP-resistant gastric cancer cell line BGC823/cDDP was induced by high-dose cDDP.The BGC-823 and BGC-823/cDDP cells were different in biological character-istics.