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通过农杆菌介导法将拟南芥抗旱基因AtGPX3导入玉米自交系郑58中,用PCR和RT-PCR法对转化玉米进行检测,在水分胁迫下对T1代转基因玉米和非转基因玉米进行抗旱性分析。结果表明,共得到56株转化苗,检测获得9个株系的30株T0代转基因阳性植株,抗性植株阳性率为53.6%。RT-PCR检测表明,T1代有6个株系为稳定遗传阳性株系,并且AtGPX3基因在转基因玉米中表达量大幅度提高。耐旱性分析表明,非胁迫条件下,非转基因和转基因株系中游离脯氨酸(Pro)和丙二醛(MDA)的含量基本无显著差异。在干旱胁迫条件下,转基因玉米叶片的Pro含量高于非转基因玉米,比非转基因株系提高了46.2%;MDA含量低于非转基因玉米,比非转基因玉米下降了34%。通过导入AtGPX3基因,可以提高玉米苗期的耐旱性。
The Arabidopsis thaliana drought resistance gene AtGPX3 was introduced into Zhengzhongzheng 58 by Agrobacterium tumefaciens-mediated method. The transformed maize was detected by PCR and RT-PCR, and the drought-tolerant T1 generation of transgenic and non-transgenic maize was under water stress Sexual analysis. The results showed that a total of 56 transformed seedlings were obtained, and 30 T0 transgenic plants positive for 9 strains were obtained. The positive rate of resistant plants was 53.6%. The results of RT-PCR showed that there were 6 lines in T1 generation with stable genetic positive lines, and the expression of AtGPX3 gene in transgenic maize significantly increased. The analysis of drought tolerance showed that under non-stress conditions, the content of free proline (Pro) and malondialdehyde (MDA) in non-transgenic and transgenic lines had no significant difference. Under drought stress, Pro content of transgenic maize leaves was higher than that of non-GM maize, 46.2% higher than that of non-GM maize leaves; MDA content was lower than non-GM maize, 34% lower than non-GM maize. Through the introduction of AtGPX3 gene, we can improve the drought tolerance of maize seedling.