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目的鉴于５Ｃ５ｃＤＮＡ（６１３ｂｐ）的２～４５０ｂｐ与ＨＳＭＲＰ１７ｍＲＮＡ（１００８ｂｐ）的５６０～１００８ｂｐ的同源性高达９５％，且后者编码的蛋白质（ＭＲＰＬ１２）也在细胞活化后表达，本研究旨在探究两者是否属同一物。方法多个人Ｂ细胞株用单抗５Ｃ５－Ｇ１作间接免疫荧光染色，激光扫描共聚焦显微镜观察。结果５Ｃ５－Ｇ１单抗识别分子表达于膜下近膜胞浆处、也可能在膜表面，但非单纯在膜上，而ＭＥＰＬ１２只表达于线粒体。另外，前Ｂ细胞Ｎａｌｍ－６与单抗５Ｃ５－Ｇ１反应阴性，活化前期Ｂ细胞Ｄａｕｄｉ和３Ｄ５的反应强阳性，分化晚期Ｂ细胞ＳＫＷ６为弱阳性。结论５Ｃ５ｃＤＮＡ与ＨＳＭＲＰ１７ｍＲＮＡ不属同一基因；单抗５Ｃ５－Ｇ１识别的分化抗原的表达在Ｂ细胞分化过程中有变化
Purpose The purpose of this study was to investigate whether the 2 ~ 450 bp of 5C5 cDNA (613 bp) and the 560 ~ 1008 bp of HSMRP17 mRNA (1008 bp) have high homology of 95% and the latter encoded protein (MRPL12) Is it the same thing? Methods Multiple human B cell line with indirect immunofluorescence staining with monoclonal antibody 5C5-G1, laser scanning confocal microscopy. Results The 5C5-G1 mAb was expressed in the submerged membrane near the cytoplasm, but also on the membrane but not on the membrane, whereas MEPL12 was only expressed in the mitochondria. In addition, pre-B cell Nalm-6 and monoclonal antibody 5C5-G1 negative reaction, pre-activated B cells Daudi and 3D5 strong reaction, differentiation of early stage B cell SKW6 is weakly positive. Conclusion 5C5 cDNA and HSMRP17 mRNA are not the same gene; monoclonal antibody 5C5-G1 recognized differentiation antigen expression in B cell differentiation process changes