目的：研究地塞米松（Ｄｅｘ）、布洛芬（Ｉｂｕ）和川芎嗪（Ｌｉｇ）对脂多糖（ＬＰＳ）诱导的肿瘤坏死因子（ＴＮＦα）表达的影响．方法：上清ＴＮＦα含量用ＥＬＩＳＡ测定；狭缝杂交对ＴＮＦαｍＲＮＡ表达量相对定量．结果：全血上清ＴＮＦα浓度随ＬＰＳ刺激剂量增加而增加，刺激后６小时ＴＮＦα达峰值．ＴＮＦαｍＲＮＡ能较早检出，ＬＰＳ刺激后２小时达峰值．Ｄｅｘ明显抑制ＬＰＳ诱导的ＴＮＦα释放，呈剂量依赖性．Ｉｂｕ和Ｌｉｇ对ＴＮＦα释放表现为双向效应．结论：Ｄｅｘ、Ｉｂｕ和Ｌｉｇ能不同程度抑制ＴＮＦα表达，可能成为严重感染的抗ＴＮＦα治疗手段． Objective: To study the effects of dexamethasone (Ix), ligustrazine (Lbu) and Lig on the expression of tumor necrosis factor (TNF) induced by lipopolysaccharide (LPS). Methods: The content of TNFα in supernatant was measured by ELISA. The expression of TNFαmRNA was quantified by slit hybridization. Results: The concentration of TNFα in whole blood supernatant increased with the increase of LPS stimulation, and reached the peak at 6 hours after stimulation. TNFα mRNA can be detected earlier, up to 2 hours after LPS stimulation. Dex significantly inhibited LPS-induced TNFα release in a dose-dependent manner. Ibu and Lig exhibit a two-way effect on TNFa release. Conclusion: Dex, Ibu and Lig can inhibit the expression of TNFα to some extent, and may be the anti-TNFα treatment for severe infection.