论文部分内容阅读
[目的]探讨人食管癌组织中Cyr61的基因表达,了解食管癌患者术后生存情况及其影响因素。[方法]运用实时荧光定量PCR技术和免疫组化染色法测定186例食管癌患者的癌组织及配对的癌旁正常食管组织中Cyr61表达水平,采用Kaplan-Meier单因素与Cox多元回归进行生存分析。[结果]食管癌组织Cyr61mRNA(7.13±4.26)表达水平显著高于癌旁正常食管组织(9.55±4.97)(P=0.000)。食管癌组织Cyr61蛋白表达水平(16.79±6.81)显著高于配对的正常食管组织(2.85±2.14)(P=0.000)。Kaplan-Meier法生存分析结果显示:性别、是否吸烟、家族史、肿瘤大小、分化程度、浸润深度、淋巴结转移、Cyr61mRNA的表达、Cyr61蛋白的表达与患者的存活率显著相关。Cox回归分析结果显示:淋巴结转移以及Cyr61蛋白的表达水平与病人的生存时间相关,相对危险度分别是1.937(95%CI1.144~3.281)和2.238(95%CI1.080~4.634)。[结论]食管癌组织中Cyr61的高表达可能影响食管癌患者术后的生存,其可作为食管癌患者预后的一个评价指标,并可能成为基因治疗的一个作用靶点。
[Objective] To investigate the gene expression of Cyr61 in human esophageal cancer and to understand the postoperative survival and its influencing factors in patients with esophageal cancer. [Method] The expression of Cyr61 in 186 cases of esophageal cancer tissues and paired paracancerous normal esophageal tissues was detected by real-time fluorescence quantitative PCR and immunohistochemical staining. Survival analysis was performed by Kaplan-Meier single factor and Cox multiple regression . [Results] The expression level of Cyr61 mRNA (7.13 ± 4.26) in esophageal cancer tissues was significantly higher than that in normal esophageal cancer tissues (9.55 ± 4.97) (P = 0.000). The expression level of Cyr61 protein in esophageal cancer tissues was significantly higher than that in matched normal esophageal tissues (16.79 ± 6.81 vs 2.85 ± 2.14, P = 0.000). Kaplan-Meier survival analysis showed that gender, smoking history, family history, tumor size, differentiation degree, depth of invasion, lymph node metastasis, Cyr61 mRNA expression and Cyr61 protein expression were significantly correlated with patient survival. Cox regression analysis showed that lymph node metastasis and the expression of Cyr61 were correlated with the survival time of patients, the relative risk were 1.937 (95% CI, 1.144 ~ 3.281) and 2.238 (95% CI1.080 ~ 4.634), respectively. [Conclusion] The high expression of Cyr61 in esophageal carcinoma may affect the postoperative survival of patients with esophageal cancer, which may be used as a prognostic indicator of esophageal cancer and may be a target of gene therapy.