【摘 要】
：
The depression of cardiac contractility induced by space microgravity is an important issue of aerospace medicine research, while its precise mechanism is still
【机 构】
：
Laboratory of Space Cellular and Molecular Biology
【基金项目】
：
国家高技术研究发展计划(863计划)
论文部分内容阅读
The depression of cardiac contractility induced by space microgravity is an important issue of aerospace medicine research, while its precise mechanism is still unknown. In the present study, we explored effects of simulated microgravity on nitric oxide (NO) level, inducible nitric oxide synthase (iNOS) expression and related regulative mechanism using electron spin resonance (ESR) spectroscopy, immunocytochemistry and in situ hybridization. We found a remarkable increase of NO level and up-regulation of iNOS and iNOS mRNA expression in rat cardiac myocytes under simulated microgravity. Staurosporine (a nonselective protein kinase inhibitor), calphostin C (a selective protein kinase C inhibitor), partially inhibited the effect of simulated microgravity. Thus regulative effect of simulated microgravity on iNOS expression is mediated at least partially via activation of protein kinase C. These results indicate that NO system in cardiac myocytes is sensitive to simulated microgravity and may play an important role in the depression of cardiac contractility induced by simulated microgravity.
其他文献
The DNA-binding activity of heat shock transcription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific to CaM
Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (Eps) inducing cell membrane pored (electroporation) are used as a means of deliv
The three-dimensional structure of Periplaneta fuliginosa densovirus (pfDNV) is determined at 2.3 nm resolution using the techniques of cryo-electron microscopy
Nuclear factor NF-κB is believed to play an important role in regulating the production of matrix met-alloproteinases (MMPs), which induce atherosclerosis, res
The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosom
Plant rubisco consists of eight large subunits (55 kD) encoded by chloroplast gene and eight small subunits (15 kD) encoded by nuclear gene. There are abundant
To explore the possibility of Schwann cells transplantation to promote the repair of injured brain stem reticular structure in rats. Methods Schwann cells origi
Lidamycin with high antitumor activity is a novel enediyne antitumor antibiotic producedby Streptomyces globisporus C1027. The 75 kb biosynthesis gene cluster o
Protoplasts from cell suspensions of young-embryo-derived calli, which were non- regenerable for long-term subculture and protoplasts from embryogenic calli
Asy (apoptosis/saibousi Yutsudo) is a novel apoptosis-inducing gene found in 1999 by Yutsudo group in Japan. In 2000, Qi Bing et al. cloned another novel gene