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目的:从人参、西洋参、竹节参、三七这4个人参属中药中提取成分,对其抗卵巢癌活性进行筛选、比较。方法:人参、西洋参、竹节参和三七分别用水和乙醇热回流提取制备相应的水提物和醇提物;上述药材70%乙醇水提取物经大孔树脂柱色谱分别制备得到总皂苷和相应的原人参二醇型皂苷和原人参三醇型皂苷部位。用噻唑蓝(MTT)法对这些提取物和部位在卵巢癌HEY细胞进行活性筛选,并用碘化丙啶(PI)染色检测提取物对细胞周期分布的影响,进一步用免疫印迹法(Western blot)检测了其对细胞周期蛋白表达的影响。划痕实验检测了提取物对细胞迁移能力的影响。结果:各提取物都表现出不同程度地抑制卵巢癌HEY细胞增殖活性,其中西洋原人参二醇型皂苷(PDSPQ)、竹节参总皂苷(TSPJ)、人参醇提物(EEPG)、人参总皂苷(TSPG)、人参原人参二醇型皂苷(PDSPG)对HEY细胞的增殖抑制比较明显,抑制率分别为45.59%,45.78%,50.48%,46.98%,64.36%,PDSPQ,TSPG,PDSPG可以不同程度的下调周期蛋白依赖性激酶(CDK)4,CDK6,细胞周期蛋白D1(cyclin D1)的表达,但对细胞迁移能力没有明显影响。结论:含有原人参二醇型皂苷的组分的抗卵巢癌HEY细胞活性比其他部位更明显,其机制可能与下调细胞周期相关蛋白CDK4,CDK6,cyclin D1的表达有关。
OBJECTIVE: To screen and compare the anti-ovarian cancer activity of four Chinese ginseng (Panax quinquefolius L.), American ginseng Methods: Ginseng, Panax quinquefolius, Panax notoginseng and Panax notoginseng were respectively extracted by water and ethanol by hot reflux extraction to prepare the corresponding water extracts and alcohol extracts. The crude extracts of 70% ethanol aqueous extracts of the medicinal materials were respectively prepared by macroporous resin column chromatography. The corresponding original ginseng diol-type saponin and the original part of the ginseng type saponin. The activity of these extracts and their fractions in ovarian cancer HEY cells was assayed by MTT method. The effect of extracts on cell cycle distribution was detected by propidium iodide (PI) staining. Western blot Its effect on cyclin expression was examined. Scratch tests examined the effect of extracts on cell migration ability. Results: All the extracts showed different degrees of inhibition on the proliferation of HEY cells. Among them, PDSPQ, TSPJ, EEPG, The inhibitory rates of TSPG and PDSPG were 45.59%, 45.78%, 50.48%, 46.98% and 64.36%, respectively. PDSPQ, TSPG and PDSPG could be different The expression of CDK 4, CDK6 and cyclin D1 was downregulated to a certain extent, but had no significant effect on cell migration ability. CONCLUSION: The anti-ovarian cancer cell line HEY containing the components of the original ginseng diol-type saponin is more obvious than other parts. The mechanism may be related to the down-regulation of the expression of CDK4, CDK6 and cyclin D1.