目的:探索罗勒组培快速繁殖技术,为罗勒种苗工厂化生产及种质资源保存提供理论依据。方法:采用组培技术,以罗勒种子萌发的无菌苗为外植体,开展丛生芽的诱导及增殖培养、壮苗培养及生根培养和移栽一系列技术研究。结果:丛生芽的诱导及增殖适宜培养基为MS+IAA 0.1 mg/L+KT 3.0 mg/L+GA32.0 mg/L;生根最佳培养基为1/2MS+IAA 0.3 mg/L+IBA 0.5 mg/L,在此条件下,根发育良好,植株健壮。结论:建立了罗勒完整的植株再生体系。 OBJECTIVE: To explore the technique of tissue culture rapid propagation of basil, and to provide theoretical basis for the factory production and germplasm conservation of basil seedlings. Methods: Using tissue culture technique, aseptic shoots germinated from basil seeds were used as explants to carry out a series of techniques of induction, proliferation, seedling growth and rooting of transplanted buds. Results: The optimum medium for inducing and multiplying buds was MS + IAA 0.1 mg / L + KT 3.0 mg / L + GA 32.0 mg / L, and the best medium for rooting was 1 / 2MS + IAA 0.3 mg / L + IBA 0.5 mg / L. Under this condition, the root developed well and the plant was robust. Conclusion: The establishment of a complete plant regeneration system basil.