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目的采用分光光度法测定角花胡颓子中总黄酮的含量。方法以芦丁为对照品,三氯化铝显色,在417 nm测定吸光度,计算角花胡颓子中总黄酮的含量。结果芦丁4.4～35.2μg.mL-1与吸光度值呈良好的线性关系,回归方程为:Y=0.043X+0.0008(r=0.9999),平均加样回收率为96.21%,RSD=1.39%(n=9)。结论所建方法简便、快速、准确,可用于角花胡颓子的质量控制。
Objective To determine the content of total flavonoids in Elaeagnus bockii by spectrophotometry. Methods Rutin was used as a reference substance and aluminum trichloride was developed. The absorbance was measured at 417 nm, and the content of total flavonoids in the Elaeagnus angustifolia was calculated. Results The linear regression equation was 4.4 ~ 35.2μg.mL-1 for rutin with the absorbance value. The regression equation was Y = 0.043X + 0.0008 (r = 0.9999). The average recoveries were 96.21% and RSD = 1.39% n = 9). Conclusion The method is simple, rapid and accurate and can be used for quality control of Elaeagnus.