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目的 :观察总丹酚酸 (Sal)对大鼠局灶性脑缺血损伤及抗氧化酶活性的影响。方法 :用插线法阻塞大鼠大脑中动脉 (MCAO) 2 4h再灌注 2h或 2 4h。梗塞灶面积用TTC染色分析 ,脑水含量用干湿重法 ,神经功能缺损采用 8级评分。并分析缺血脑组织的 4种抗氧化酶活性水平。结果 :Sal 5 .0 ,10 .0mg/kg(于MCAO术后 10min及 2 4h由舌下静脉注射给药 )可明显缩小脑梗塞面积 ,改善神经功能缺损 ,并减少脑水含量。大鼠MCAO 2 4h再灌注初期 (2h)时 ,脑组织超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH PX)和过氧化氢酶 (CAT)的活性明显升高 ,谷胱甘肽S 转移酶 (GSH ST)的活性显著下降。而在再灌注后期 (2 4h) ,则SOD ,GSH PX及CAT活性显著下降 ,GSH ST的活性进一步降低。Sal可在缺血再灌注状态下进一步升高SOD ,GSH PX及CAT活性 ,但对GSH ST的水平无明显影响。结论 :Sal对大鼠局灶性脑缺血损伤具有神经保护作用 ,此作用可能与SOD ,GSH PX及CAT活性水平的升高有关
Objective: To observe the effect of salvianolic acid on focal cerebral ischemic injury and antioxidant enzyme activity in rats. Methods: The middle cerebral artery (MCAO) of rats were occluded for 24 hours and then reperfused for 2 hours or 24 hours. Infarct area using TTC staining analysis, brain water content using wet and dry method, neurological deficit using 8 grade score. The levels of four antioxidant enzymes in ischemic brain tissue were analyzed. Results: Sal 5 .0, 10 .0 mg / kg (10 min after MCAO and 24 h by sublingual intravenous injection) can significantly reduce the infarct size, improve neurological deficits and reduce brain water content. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH PX) and catalase (CAT) in brain tissue of MCAO 2 4 h group increased significantly at 2 h after reperfusion, Glutathione S-transferase (GSH ST) activity was significantly reduced. However, at the later stage of reperfusion (24 h), the activity of SOD, GSH PX and CAT decreased significantly, and the activity of GSH ST decreased further. Sal could further increase the activity of SOD, GSH PX and CAT under the condition of ischemia-reperfusion, but had no significant effect on the level of GSH-ST. Conclusion: Sal has neuroprotective effect on focal cerebral ischemia in rats, which may be related to the increase of SOD, GSH PX and CAT activity