将狂犬病病毒７ａＧ固定毒适应于Ｖｅｒｏ细胞，建立了稳定的ＶＲＧ适应株。病毒滴度可达７．６９ｌｏｇＬＤ５０／ｍｌ，病毒最适增殖时间５—７天，最适种毒比例１：１０￣３。应用转瓶培养Ｖｅｒｏ细胞增殖病毒，收获病毒液经β—丙内脂灭活，超离浓缩制备疫苗，效力试验结果（ＮＩＨ法）较原制疫苗成倍增高，且都大于２．５ＩＵ／２ｍｌ，表明ＶＲＧ适应株可应用于制备Ｖｅｒｏ细胞狂犬病疫苗。 Rabies virus 7aG fixed poison adapted Vero cells, the establishment of a stable VRG adaptive strain. Virus titers up to 7.69logLD50 / ml, the optimal virus propagation time of 5-7 days, the most appropriate ratio of 1: 10 ~ 3 poisoning. Vero cell proliferation virus was harvested by roller flask, the virus solution was inactivated by β-propiolactone, and the vaccine was prepared by ultracentrifugation. The efficacy test results (NIH method) were multiplied more than the original vaccine and both were more than 2.5IU / 2ml , Indicating that the VRG adapted strain can be used for preparing Vero cell rabies vaccine.