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研究白介素17(interleukin 17,IL-17)在药物难治性颞叶外侧型癫痫(lateral temporal lobe epilepsy,LTLE)病灶中的表达和分布,探讨IL-17在TLE发生发展中可能的作用。使用real-time PCR、Western blotting、免疫组化及荧光双标检测12例LTLE致痫灶及8例正常对照脑组织中IL-17的表达。结果提示:IL-17在LTLE中的mRNA和蛋白表达水平较对照组明显增高(P<0.05),且IL-17高表达于LTLE的神经元、星形胶质细胞以及血管内皮细胞。上述研究结果提示,IL-17可能参与了TLE的发生发展。
To investigate the expression and distribution of interleukin 17 (IL-17) in the lesions of temporal lobe epilepsy (LTLE) and explore the possible role of IL-17 in the development and progression of TLE. Real-time PCR, Western blotting, immunohistochemistry and fluorescent double labeling were used to detect the expression of IL-17 in 12 cases of LTLE and 8 cases of normal brain tissue. The results showed that IL-17 mRNA and protein expression in LTLE was significantly higher than that in the control group (P <0.05), and IL-17 was highly expressed in neurons, astrocytes and vascular endothelial cells of LTLE. The above results suggest that IL-17 may be involved in the occurrence and development of TLE.