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目的:建立牛眼小梁细胞体外培养体系,了解小梁细胞的形态与功能,探讨房水引流阻力在原发性开角型青光眼(primary open angle glaucoma,POAG)的病因和发病机制中的作用;对小梁细胞进行药物实验以指导临床用药和开发新的抗青光眼药物.方法:牛眼小梁网来源于20只处死的小牛眼,小梁细胞原代和传代培养;用光镜、电镜观察原代和传代牛眼小梁细胞的生长特性和形态特征;用放射自显影和流式细胞术等分析传3代和传10代细胞的细胞动力学;以及抗青光眼药物0.25mg.ml-1肾上腺素(epinephrine,EPI)和0.025Mg.ml-1地匹福林(dipivalyl epinephrine,DPE)对传3代细胞的细胞动力学影响.结果:培养的牛眼小梁细胞生长特性和形态特征相似于人眼小梁细胞,其生长类型多数为上皮细胞型,少数为成纤维细胞型;随着传代的增加.细胞微丝含量减少,DNA合成时间(Ts)和细胞周期时间(Tc)明显延长;抗青光眼药物EPI(0.25mg.ml-1)和DPE(0.025mg.ml-1)使微丝解聚,Ts和Tc明显延长.结论:建立牛眼小梁细胞体外培养方法,研究该细胞形态、功能和药理作用,为研究人眼小梁细胞、探索POAG的病因和发病机制提供重要资料;抗青光眼药物EPI(0.25mg.ml-1)和DPE(0.025mg.ml-1)在该浓度情况下对传3代小梁细胞的微丝和细胞动力学产生明显影?“,”Objective:To establish the method of culturing calf trabecular cells (CTCs) in vitro, to understand the morphology and function of CTCs, to probe into the effect of resistance of aqueous outflow in the pathogenesis and mechanism about primary open angle glaucoma(POAG). To direct the clinical using of drugs and to open up new antiglau-comatous medicine by pharmacological studies of CTCs.Methods :Trabecular meshwork was collected from twenty eyeballs of calf donors after slaughter. The tiusse was primarily cultured and cells were subcultured. The growing characteritics and morphological features of cultured primary and passaged cells were observed by light and electron microscopes. Cell kinetics of the third and tenth passaged cells were analysed using autoradiography and flow cytometry. The influence of the antiglaucomatous drugs 0. 25mg.ml-1 epinephrine (EPI) and 0.025mg.ml-1 dip-ivalyl epinephrine (DPE) on cell kinetics of the third passaged cells was studied. Results:The growing characteritics and morphological features of cultured CTCs were as same as those of human trabecular cells. Growing types of CTCs included most of epitheial cell and few of fibroblast. The amount of cellular microfilaments was reduced, DNA synthesis time(Ts) and cell cycle time(Tc) were obviously prolonged with passaged increasing. Antiglaucomatous drugs-EPI(0. 25mg.ml-1 ) and DPE (0.025mg.ml-1) made microfilaments dissolving, Ts and Tc obviously prolonging. Conclusion: Establishing the method of culturing CTCs in vitro and understanding their morphology, function and pharmacological effects provided an important information for studying human trabecular cells and probing into the effect of resistance of aqueous outflow in the pathogenesis and mechanism about POAG. These studies indicated that antiglaucomatous drugs-EPI (0. 25mg. ml -1) and DPE (0.025mg. ml -1 ) influenced obviously microfilaments and cell kinetics of the third passaged cells and suggested that it is not to be ignored that 1%EPI and 0.1%DPE may make CTCs' microfilaments dissolving and may inhibit CTCs' division and proliferation when they are used in clinical therapy. Eye Science 1999; 15; 97 - 102.