DCPTA对盐胁迫下玉米苗期根系生长、渗透调节及膜透性的影响

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黑龙江省西部地区盐碱土面积约有66.7×104hm2,制约着黑龙江省粮食生产水平的进一步提高。叔胺类活性物质2-(3,4-二氯苯氧基)-乙基-二乙胺(DCPTA)具有提高作物抗逆性作用,为探讨DCPTA提高玉米耐盐性机制,采用营养液水培试验,在前期试验基础之上,选取“先玉335”(耐盐性强)和“丰禾1号”(耐盐性弱)2个品种,研究DCPTA(15mg·L-1)对Na Cl胁迫下(150 mmol·L-1)玉米幼苗根系生长、渗透调节及质膜透性的影响。结果表明:DCPTA能增加盐胁迫下幼苗的根长、根表面积、根体积、根鲜重和根干重,缓解盐胁迫对根系生长的抑制;DCPTA维持了根系水分平衡,提高了盐胁迫下幼苗根系相对含水量,“先玉335”和“丰禾1号”分别提高3.6%和6.4%;DCPTA通过提高根系可溶性糖含量和可溶性蛋白含量,降低幼苗根系脯氨酸含量,增强了盐胁迫下玉米幼苗根系的渗透调节能力,并通过降低根系丙二醛(MDA)含量和相对电导率,保护了根系质膜结构和功能;与盐胁迫相比,“先玉335”丙二醛含量和相对电导率分别降低21.6%和24.2%,“丰禾1号”分别降低28.1%和30.4%;DCPTA缓解盐胁迫对根系伤害的效果表现为“丰禾1号”>“先玉335”。 The area of ​​saline-alkali soil in the western Heilongjiang Province is about 66.7 × 104hm2, which restricts the further improvement of grain production in Heilongjiang Province. Tertiary amine active substance 2- (3,4-dichlorophenoxy) -ethyl-diethylamine (DCPTA) has the effect of improving plant stress resistance. To explore the mechanism of DCPTA improving salt tolerance of maize, In order to study the effects of DCPTA (15mg · L-1) on the basis of previous experiments, two varieties, Xianxian 335 (strong salt tolerance) and Fenghe 1 (weak salt tolerance) 1) on root growth, osmotic adjustment and plasma membrane permeability of maize seedling under NaCl stress (150 mmol·L-1). The results showed that DCPTA could increase the root length, root surface area, root volume, root fresh weight and root dry weight of seedlings and alleviate the inhibition of root growth under salt stress. DCPTA maintained the root water balance and increased the seedling growth under salt stress Root relative water content increased by 3.6% and 6.4% respectively in Xianxu 335 and Fenghe 1; DCPTA decreased the content of proline in roots and increased the contents of soluble sugar and soluble sugar Under osmotic stress, osmotic adjustment ability of maize seedling root system was inhibited, and the structure and function of the plasma membrane of the root were protected by reducing the malondialdehyde (MDA) content and relative conductivity of the root system. Compared with the salt stress, Malondialdehyde content and relative electric conductivity decreased by 21.6% and 24.2%, respectively, and “Fenghe 1” decreased by 28.1% and 30.4%, respectively. DCPTA alleviated the effect of salt stress on root injury as “Fenghe 1 ”“ ”First jade 335 ".
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