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目的建立HPLC法同时测定参茸安神片中远志酮Ⅲ、3,6’-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B和五味子醇甲的方法。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈–0.1%磷酸溶液,梯度洗脱;检测波长:320 nm(0~16 min检测远志酮Ⅲ、3,6’-二芥子酰基蔗糖)、210 nm(16~24 min检测细叶远志皂苷、远志皂苷B)、250 nm(24~35 min检测五味子醇甲);体积流量:1.0 m L/min;柱温:30℃;进样量为10μL。结果远志酮Ⅲ、3,6’-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B和五味子醇甲分别在3.85~77.00、6.22~124.40、6.57~131.40、7.61~152.20、4.96~99.20μg/m L线性关系良好(r≥0.999 2);平均回收率分别为98.17%、99.24%、99.90%、97.63%、96.83%,RSD值分别为1.14%、1.23%、0.70%、1.52%、0.81%。结论本法稳定可靠、简便易行,为全面控制参茸安神片的质量提供了参考。
OBJECTIVE To establish a HPLC method for the simultaneous determination of mifepristone Ⅲ, 3,6’-aescinylsucrose, Prunella sibiricum, Tenebrio saponins B and Schizandrol A in ginseng and antiepileptic tablets. Methods Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution. The detection wavelength was 320 nm (0-16 min) Digestible acyl sucrose), 210 nm (16 ~ 24 min detection of Prunus Sapindus saponin, teigenin B), 250 nm (24 ~ 35 min detection Schisandra alcohol); volume flow rate: 1.0 m L / min; ℃; injection volume of 10μL. Results Polygalacetin Ⅲ, 3,6’-diaminoacylsuccinose, Sapindus saponins, teigenin B and schizandrin were respectively in the range of 3.85-77.00,6.22-124.40,6.57-131.40,7.61-152.20,4.96-99.20μg / The average recovery was 98.17%, 99.24%, 99.90%, 97.63% and 96.83%, respectively, with RSD values of 1.14%, 1.23%, 0.70%, 1.52% and 0.81% . Conclusion This method is stable and reliable, simple and easy to provide a reference for the comprehensive control of the quality of ginseng and soothing tablets.