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利用IPTG诱导大肠杆菌ST-LTB融合基因表达,获取了大量包涵体蛋白并进行纯化。用PEG-400作为载体材料,辅以蜂胶和span-60等佐剂包裹ST-LTB融合蛋白,制备成PEG-400复合佐剂纳米疫苗,并使用透射电镜观察所制得纳米粒的形态,用考马斯亮蓝G-250法检测其载药率。结果表明:电镜观察所制得的纳米粒为球形,粒径在55-75nm之间分布。PEG-400复合佐剂纳米疫苗抗原载药率为73.22±0.21%,符合纳米疫苗的工艺要求。
IPTG was used to induce E. coli ST-LTB fusion gene expression, a large number of inclusion bodies were obtained and purified. PEG-400 as a carrier material, supplemented with propolis and span-60 and other adjuvants wrapped ST-LTB fusion protein preparation of PEG-400 complex adjuvant nano-vaccine, and the use of transmission electron microscopy to observe the morphology of the resulting nanoparticles with Coomassie Brilliant Blue G-250 test its drug-loading rate. The results showed that the nanoparticles prepared by electron microscopy were spherical and their particle size distribution was between 55 and 75 nm. PEG-400 composite adjuvant nano-vaccine antigen loading rate of 73.22 ± 0.21%, in line with the requirements of the nano-vaccine process.