The rutting resistance of multilayer asphalt overlay was researched by using laboratory wheel tracking test. The effects of loading level and test temperature on rutting resistance of asphalt overlay structure were evaluated by means of multilayer specimens .In comparison with multilayer tests, standard specimens of various layers were also conducted to evaluate the rutting resistance. Experimental results indicated that the test temperature and applied load have a significant effect on rutting resistance of asphalt concrete. Higher test temperature and heavier applied load resulted in higher rut depths. In addition, the mutilayer wheel tracking test has been demonstrated to be a more reasonable solution in evaluation on rutting resistance of asphatt pavement structure beasuse it reflects the cumulative permanent deformation in all of asphalt layers.