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目的研究与三氧化二砷(As_2O_3)具有协同效应治疗胰腺癌的药物。方法以胰腺癌细胞系SW1990为研究对象,观察5-氟尿嘧啶(5-Fu)、健择(Gemcitabine)和全反式维甲酸(AT- RA)与As_2O_3共同作用对细胞的影响。通过台盼蓝拒染法检测细胞生长和细胞活力,流式细胞仪检测Annexin V或PI阳性细胞的含量,评价以上药物对细胞增殖和凋亡的作用。结果5-Fu和Gemcitabine与As_2O_3无协同效应。单独应用As_2O_3或ATRA均抑制SW1990细胞生长,不诱导细胞凋亡。其中,对照组活细胞密度为(8.5±0.3)×10~5/ml,As_2O_3组为(4.4±0.1)×10~5个/ml,ATRA组为(6.7±0.2)×10~5个/ml。但是,As_2O_3和ATRA共同处理SW1990细胞后,细胞生长明显抑制,并诱导细胞凋亡。对照组活细胞密度为(8.5±O.3)×10~5个/ml,As_2O_3+ATRA组为(3.3±0.1)×10~5个/ml;对照组细胞活力为(92.0±1.2)%,As_2O_3组为(90.0±1.3)%,ATRA组为(93.0±1.4)%,As_2O_3+ATRA组为(65.0±2.1)%;对照组Annexin V和PI阳性细胞的含量为(6.0±1.2)%,As_2O_3组为(11.0±3.3)%,ATRA组为(5.0±1.4)%,As_2O_3+ATRA组为(37.0±5.3)%。结论As_2O_3和ATRA可协同诱导胰腺癌细胞凋亡,两者联合应用可能作为胰腺癌辅助治疗的另一选择。
Objective To study the synergistic effect of arsenic trioxide (As_2O_3) in the treatment of pancreatic cancer. Methods Pancreatic cancer cell line SW1990 was used to investigate the effect of 5-Fu, Gemcitabine and AT-RA on As2O3. The cell growth and cell viability were detected by trypan blue exclusion. The contents of Annexin V or PI positive cells were detected by flow cytometry. The effects of the above drugs on cell proliferation and apoptosis were evaluated. Results 5-Fu and Gemcitabine had no synergistic effect with As 2 O 3. As2O3 or ATRA alone inhibited SW1990 cell growth and did not induce apoptosis. In the control group, the viable cell density was (8.5 ± 0.3) × 10-5 / ml in As2O3 group and (4.4 ± 0.1) × 10-5 / ml in As2O3 group and 6.7 ± 0.2 × 10-5 / ml. However, As2O3 and ATRA co-treated SW1990 cells, cell growth was significantly inhibited, and induce apoptosis. The cell viability in control group was (8.5 ± 0.3) × 10 ~ 5 cells / ml and that in As2O3 + ATRA group was (3.3 ± 0.1) × 10 ~ 5 cells / ml. The cell viability in control group was (92.0 ± 1.2)% (90.0 ± 1.3)% in As2O3 group, (93.0 ± 1.4)% in ATRA group and (65.0 ± 2.1)% in A2O3 group and (6.0 ± 1.2)% in control group (11.0 ± 3.3)% in As2O3 group, (5.0 ± 1.4)% in ATRA group and (37.0 ± 5.3)% in As2O3 + ATRA group. Conclusion As 2 O 3 and ATRA can synergistically induce the apoptosis of pancreatic cancer cells, and the combination of them may be used as an adjunct therapy for pancreatic cancer.