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目的探讨慢性乙型肝炎患者外周血CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+和CD8+CD45RO+T淋巴细胞亚群的特点及其与肝病病情的关系.方法采集46例轻中度慢性乙型肝炎患者、58例重度慢性乙型肝炎患者和30例健康人的外周抗凝血,应用流式细胞技术三色荧光分析法对其外周血中CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+和CD8+CD45RO+T淋巴细胞亚群进行检测.结果轻中、重度慢性乙型肝炎患者与正常人相比,其外周血中CD4+,CD8+T细胞均无明显改变;CD8+CD45RA+T细胞均明显降低,CD8+CD45RO+T细胞均明显增高,而CD4+CD45RA+,CD4+CD45RO+T细胞均无明显改变;重度慢性乙型肝炎患者与轻中度慢性乙型肝炎患者相比,CD8+CD45RA+T细胞明显降低(P<0.05),CD8+CD45RO+T细胞明显升高(P<0.05).结论乙型肝炎慢性化过程中,CD8+CD45RO+T细胞起重要作用且与慢性乙型肝炎患者病情的进展呈正相关;检测CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+和CD8+CD45RO+T淋巴细胞亚群比检测CD4+和CD8+T细胞亚群能使我们更加正确、充分、全面地了解慢性乙型肝炎的发病机制和预后,从而有效地指导临床治疗.
Objective To investigate the characteristics of CD4 + CD45RA +, CD4 + CD45RO +, CD8 + CD45RA + and CD8 + CD45RO + T lymphocyte subsets in patients with chronic hepatitis B and their relationship with the severity of liver disease.Methods 46 cases of mild to moderate chronic type B Hepatic patients, 58 patients with severe chronic hepatitis B and 30 healthy people were measured by flow cytometry. The levels of CD4 + CD45RA +, CD4 + CD45RO +, CD8 + CD45RA + and CD8 + + CD45RO + T lymphocyte subsets.Results There was no significant change of CD4 +, CD8 + T cells in peripheral blood of mild to moderate-severe chronic hepatitis B patients compared with normal controls, and CD8 + CD45RA + T cells were significantly increased CD45RA + T cells in CD4 + CD45RA + and CD4 + CD45RO + T cells were significantly decreased in patients with severe chronic hepatitis B as compared with patients with mild to moderate chronic hepatitis B (P <0.05), and the percentage of CD8 + CD45RO + T cells was significantly increased (P <0.05) .Conclusion CD8 + CD45RO + T cells play an important role in the process of chronic hepatitis B and play an important role in the pathogenesis of chronic hepatitis B The progression of the disease was positively correlated; CD4 + CD45RA +, CD4 + CD45RO +, CD8 + CD45RA + and CD8 + CD45RO + T lymphocytes were detected We are more correct, than the subsets fully detect CD4 + and CD8 + T cell subsets can fully understand the pathogenesis of chronic hepatitis B and prognosis, so as to effectively guide the clinical treatment.