分泌免疫球蛋白杂交瘤的克隆筛选,一般需经三个步骤,即(1)杂交瘤培养物的生长;(2)识别培养物分泌的所需抗体;(3)特异性细胞株的克隆。本文报告了一步法筛选的方法,即融合细胞悬于DMEM+20%FCS中,与等量0.5%琼脂糖DMEM+20%FCS+HAT2X混合,铺在预先培养有鼠胚成纤维细胞株STO的平碟中培养。10～12天后该平碟加入42℃溶于DMEM的0.75%琼脂 Clonal screening of secreted immunoglobulin hybridomas typically takes three steps, namely (1) the growth of a hybridoma culture; (2) the identification of the desired antibodies secreted by the culture; and (3) the cloning of specific cell lines. This article reports a one-step screening method in which the fusion cells are suspended in DMEM + 20% FCS, mixed with an equal amount of 0.5% agarose DMEM + 20% FCS + HAT2X and plated on preincubated STO Flat dish in training. After 10 to 12 days, add the plate to 0.75% agar in DMEM at 42 ° C