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目的:了解石河子地区手足口病病原学流行特征,为本地区手足口病的防控提供科学依据。方法:对2011-2012年石河子市第一附属医院儿科及石河子各团场医疗机构的手足口病病例采集粪便标本,提取核酸用逆转录-聚合酶链反应(PCR法)对肠道通用病毒、Ev71、CoxA16核酸进行检测。结果:120份手足口病临床诊断病例的粪便标本中,其中88份肠道通用病毒核酸阳性,检出率为73.33%,CoxA16的阳性检出率为29.17%,Ev71的阳性检出率为16.67%,且CoxA16的阳性检出率与Ev71的阳性检出率差异意义有统计学(X2=5.31,p<0.05)。发病以6月高发为主,不同性别间病毒核酸的检出率无统计学差异,0~5儿童为高发人群。结论:CoxA16是石河子地区2011-2012年手足口病的主要病原体,今后应加强手足口病的病原学检测,进一步掌握手足口病的流行规律。
Objective: To understand the epidemiological features of HFMD in Shihezi area and to provide a scientific basis for the prevention and control of HFMD in this area. Methods: Stool samples were collected from the Pediatrics Department of the First Affiliated Hospital of Shihezi City and the medical institutions of Shihezi Medical Field from 2011 to 2012, and nucleic acid was extracted for reverse transcription - polymerase chain reaction (PCR) Ev71, CoxA16 nucleic acid. Results: Of the 120 cases of hand-foot-mouth disease clinically diagnosed cases of faecal samples, 88 of which were positive for enteric virus nucleic acid, the detection rate was 73.33%, CoxA16 positive detection rate was 29.17%, Ev71 positive detection rate was 16.67 %, And the difference between CoxA16 positive rate and Ev71 positive rate was statistically significant (X2 = 5.31, p <0.05). The incidence of high prevalence in June, the detection rate of nucleic acid between different sex no significant difference, 0 to 5 children are high incidence of the crowd. Conclusion: CoxA16 is the major pathogen of hand-foot-mouth disease in Shihezi area from 2011 to 2012. Pathogen detection of hand-foot-mouth disease should be strengthened in the future to further understand the prevalence of HFMD.