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在同一受精卵中,同时研究三系育性遗传物质之间的相互作用及其表达情况,对于植物雄性不育机理的认识是重要的,如用一般的“(不育系×保持系)×恢复系”的方式是不能实现的。我们采用了把失去受精能力的高粱恢复系5号花粉匀浆涂于母本不育系3197A的柱头上,然后授以保持系3197B的新鲜花粉的方法,使恢复系花粉匀浆参与不育系×保持系的授粉过程,成功地获得了具有3197A、3197B和恢复系5号三者遗传特性的后代,达到了恢复系雄性可育基因通过非配子融合转移的目的。本试验不但为研究植物雄性不育的本质和寻找父本花粉恢复基因的载体创造了条件,而且在育种方法上,为转移某些特异的有益基因,开辟了一条新途径。
In the same fertilized egg, it is important to study the interaction and expression between three lines of fertile genetic material at the same time, and understand the mechanism of plant male sterility. For example, using the general “(sterile line × maintainer line) × Restore system ”approach can not be achieved. We used the sorghum restorer line No.5 pollen homogenate which had been lost to fertilization to the stigma of male sterile line 3197A and then fed the fresh pollen of line 3197B to make the restorer pollen homogenate participate in the CMS line × to maintain the pollination process of the lines, the progeny with inheritance of 3197A, 3197B and restorer line No.5 were successfully obtained, and the purpose of the transfer of the male-fertile gene of restoring line through non-gamete fusion was achieved. This experiment not only provided the conditions for the study of the nature of plant male sterility and the vector for finding pollen-restoring genes of paternal parents, but also opened up a new way to transfer some specific beneficial genes in breeding methods.