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构建一种反义抗细胞凋亡基因-正义促细胞凋亡基因相连结的新型表达质粒,以探讨其对细胞凋亡的影响。用分子克隆方法,成功地构建了反义bcl-2-正义bcl-Xs逆转录病毒重组体,初步结果显示此重组体比反义bcl-2或正义bcl-Xs逆转录病毒重组体具有更强的促细胞凋亡作用。该新型表达质粒的构建为探讨细胞凋亡提供了新工具,新思路。
To construct an antisense anti-apoptotic gene - sense expression of apoptosis-promoting gene linked to a new plasmid to explore its impact on apoptosis. The recombinant cloning of antisense bcl-2-sense bcl-Xs retrovirus was successfully constructed by molecular cloning method. The preliminary results showed that the recombinant retroviral bcl-2 was more potent than antisense bcl-2 or bcl-Xs recombinant retroviral vector Pro-apoptotic effect. The construction of the new expression plasmid provides a new tool and a new idea for exploring apoptosis.