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目的探讨青蒿琥酯(artesunate,Art)对人子宫内膜癌细胞血管生成素-2(angiopoietin-2,ang-2)基因表达及细胞增殖的影响。方法2007年12月至2008年8月于南京大学医学院附属鼓楼医院实验室培养人子宫内膜癌ishikawa细胞株,利用RT-PCR方法检测不同浓度Art对ishikawa细胞ang-2mRNA基因表达的影响;Westernblot方法检测Art对ang-2蛋白表达的影响,四甲基偶氮唑蓝(MTT)法测定Art对ishikawa细胞增殖的影响;荧光显微镜观察ishikawa细胞形态学的变化;transwell实验检测Art对ishikawa细胞侵袭能力的影响。结果人子宫内膜癌ishikawa细胞ang-2mRNA基因及其蛋白质表达水平均显著下降(P<0.01);增殖被抑制(P<0.01),抑制率达70.25%;部分ishikawa细胞形态改变,甚至形成凋亡小体;侵袭能力明显下降(P<0.01)。结论Art能抑制ang-2表达,同时抑制ishikawa细胞增殖,促进凋亡。
Objective To investigate the effect of Artesunate (Art) on angiopoietin-2 (ang-2) gene expression and cell proliferation in human endometrial carcinoma cells. Methods From December 2007 to August 2008, ishikawa cells were cultured in the laboratory of Drum Tower Hospital affiliated to Nanjing University Medical College. The effects of different concentrations of Art on ang-2 mRNA expression in ishikawa cells were detected by RT-PCR. Western blotting was used to detect the effect of Art on the expression of ang-2 protein. The effect of Art on the proliferation of ishikawa cells was detected by MTT assay. The morphological changes of ishikawa cells were observed by fluorescence microscopy. The effect of Art on ishikawa cells Influence of invasion ability. Results The mRNA and protein expression levels of ang-2 in ishikawa cells were significantly decreased (P <0.01), the proliferation was inhibited (P <0.01), and the inhibitory rate was 70.25%. Some ishikawa cells changed and even formed Death bodies; Invasive ability decreased significantly (P <0.01). Conclusion Art can inhibit ang-2 expression and inhibit ishikawa cell proliferation and apoptosis.